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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation

Fig. 5

source was first run until steady-state values were reached. Using these steady-state values as initial conditions, we then performed a second run with starch as the main carbon source. a Steady-state growth rate of an average cell co-expressing α-amylase and glucoamylase. b Steady-state growth rate of an average cell in the consortium. Cell A expresses α-amylase, while Cell B expresses glucoamylase. The consortium growth rate is calculated as the average growth rate of the two cells. c Comparison of monoculture and consortium growth rate (difference measured as the average growth rate of the consortium minus the monoculture growth rate)

Changes in the steady state growth rate with varying transcription rates for α-amylase (ea) and glucoamylase (eb), in monoculture and division of labour (DOL) systems of expression. Changes in growth rate (λ, min−1) for an average cell in a monoculture or a two-strain consortium, as the maximum transcription rates of α-amylase and glucoamylase (ωea and ωeb respectively) are varied. To obtain biologically realistic values for each parameter combination, a numerical simulation with glucose as the main carbon

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