Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Oral vaccination with recombinant Lactobacillus casei expressing Aha1 fused with CTB as an adjuvant against Aeromonas veronii in common carp (Cyprinus carpio)

Fig. 3

Expression of Aha1 protein from recombinant L. casei cell lysate by Western blot analysis and expression of Aha1 protein on recombinant cell surface by fluorescence microscopy analysis. A Identification of the proteins of expressed by the recombinant strains pPG-Aha1/Lc CC16 and pPG-Aha1-CTB/Lc CC16 by Western blotting with mouse anti-Aha1. MW indicates the molecular mass markers (kDa). 1: The expression of induced pPG-Aha1/Lc CC16; 2: The expression of induced pPG-Aha1-CTB/Lc CC16; 3: The expression of induced pPG/Lc CC16. B fluorescence microscopy analysis of Aha1 protein expression on recombinant cell surface. pPG-Aha1/Lc CC16 (left), pPG-Aha1-CTB/Lc CC16 (middle) and pPG/Lc CC16 (right), magnification: × 1000. There was green fluorescence on the surface of pPG-Aha1/Lc CC16 and pPG-Aha1-CTB/Lc CC16, respectively. As controls, no immunofluorescence reaction on the pPG/Lc CC16 cell surface

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us