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Fig. 12 | Microbial Cell Factories

Fig. 12

From: Oral vaccination with recombinant Lactobacillus casei expressing Aha1 fused with CTB as an adjuvant against Aeromonas veronii in common carp (Cyprinus carpio)

Fig. 12

The identification of colonization ability of recombinant L. casei in the different intestinal sections of fish. The single colonies were randomly selected and conducted to PCR using pPG-vector specific primers (upper images of each group) and the housekeeping gene dnaA primers (lower images of each group). Bands indicated with orange (1250 bp), purple (2288 bp), black (2699 bp) and red arrows (615 bp) were further analyzed by DNA sequencing and were in concert with the putative sequences. M: DNA ladder (bp), Lane 1–5: PCR product of recombinant L. casei using specific primers

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