Table 1 Strains used in this study
From: Engineered Bacillus subtilis for the de novo production of 2′-fucosyllactose
Strains | Characterization | Source |
|---|---|---|
B. subtilis 168 | Standard strain, starting strain | lab stock |
E. coli DH5α | Cloning host | lab stock |
E. coli BL21 (DE3) | Standard strain | lab stock |
BS | B. subtilis 168, PxylA-comK::comK | lab stock |
BS0 | BS, P43-manB, P43-manC, P43-gmd, P43-wcaG, P43-futC | this work |
BS-MB | BS0-pP43NMK-manB-His | this work |
BS-MC | BS0-pP43NMK-manC-His | this work |
BS-GD | BS0-pP43NMK-gmd-His | this work |
BS-WG | BS0-pP43NMK-wcaG-His | this work |
BS1 | BS0, P43-manB, P43-gmd, P43-wcaG, P43-futC | this work |
BS2 | BS1, PxylA-manC | this work |
BS3 | BS2, ΔyesZ | this work |
BS4 | BS2, ΔganA | this work |
BS5 | BS2, ΔyesZ, ΔganA | this work |
BS6 | BS5, Pnative-ndk | this work |
BS7 | BS5, PxylA-ndk | this work |
BS8 | BS5, Phbs-ndk | this work |
BS9 | BS5, P43-ndk | this work |
BS10 | BS8, Pnative-yvyI | this work |
BS11 | BS8, P43-yvyI | this work |
BS12 | BS8, Pnative-manA | this work |
BS13 | BS8, P43-manA | this work |
BS14 | BS12, P1-manC:: PxylA-manC | this work |
BS15 | BS12, P2-manC:: PxylA-manC | this work |
BS16 | BS12, P3-manC:: PxylA-manC | this work |
BS17 | BS12, P4-manC:: PxylA-manC | this work |
BS18 | BS12, P5-manC:: PxylA-manC | this work |
BS19 | BS12, P6-manC:: PxylA-manC | this work |
BS20 | BS12, P7-manC:: PxylA-manC | this work |
BS21 | BS20, P7-manA::Pnative-manA | this work |