Fig. 6
From: Engineered Bacillus subtilis for the de novo production of 2′-fucosyllactose
Engineering manA transcriptional regulation system to promote continuous synthesis of 2′-FL. A Fed-batch fermentation of strain BS20 in a 3-L bioreactor. 2′-FL synthesis stopped after 12 h fermentation. B The original promoter of manA was replaced to promote continuous 2′-FL production. qRT-PCR results showed that the expression level of the manA gene controlled by the constitutive promoter at 24 h was 2.297 times higher than that at 12 h. All data were the average of three independent studies with standard deviations