Strains/Plasmids | Relevant characteristic | Sources |
---|---|---|
Strains E. coli | ||
 Trans10 | F− mcrA, Δ(mrr-hsdRMS-mcrBC), φ80, lacZΔM15, ΔlacX74, recA1, araΔ139, Δ(ara-leu)7697, galU, galK, rpsL(StrR), endA1, nupG | Transgen Biotech |
P. pastoris | ||
 GS115 | his4 - | Invitrogen |
 JQ01 | Derived from GS115, the promoter of IPS gene was replaced with pGAP | This work |
 JQ02 | Derived from JQ01, with PpITR1 and PpITR2 genes deleted | This work |
 JQ03 | Derived from JQ02, with pfk2 gene deleted | This work |
 JQ04 | Derived from JQ03, overexpression of ScIPS gene from S. cerevisiae and EcIMP gene from E. coli | This work |
 JQ05 | Derived from JQ04, the promoter of zwf gene was replaced with pGUT1 | This work |
 JQ06 | Derived from JQ04, the promoter of pgi gene was replaced with pGUT1 | This work |
 JQ07 | Derived from JQ04, the promoters of pgi and zwf genes were replaced with pGUT1 | This work |
 JQ08 | Derived from JQ07, the promoter of pfk1 gene was replaced with pGUT1 | This work |
Plasmids | ||
 pGAPZ A | ColE1 ori, ZeoR, pGAP | Invitrogen |
 pPIC9K | ColE1 ori, pAOX1 promoter, PpHIS4, AmpR, KanR | Invitrogen |
 pEASY-T3 | ColE1 ori, AmpR, TA cloning vector | Transgen Biotech |
 pJQ | pEASY-T3 derivative; ZeoR, pAOX-mazF | This work |
 pJQ01 | pJQ derivative for markerless replacing the promoter of IPS gene with pGAP | This work |
 pJQ02-T1 | pJQ derivative for markerless deletion of possible inositol transporter gene PpITR1 | This work |
 pJQ02-T2 | pJQ derivative for markerless deletion of possible inositol transporter gene PpITR2 | This work |
 pJQ03 | pJQ derivative for markerless deletion of pfk2 gene | This work |
 pJQ04 | pJQ derivative for markerless knock‑in of ScIPS and EcIMP expression cassettes in the PpITR1 locus | This work |
 pJQ05 | pJQ derivative for markerless replacing the promoter of zwf gene with pGUT1 | This work |
 pJQ06 | pJQ derivative for markerless replacing the promoter of pgi gene with pGUT1 | This work |
 pJQ08 | pJQ derivative for markerless replacing the promoter of pfk1 gene with pGUT1 | This work |