Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism

Strains/Plasmids

Relevant characteristic

Sources

Strains E. coli

 Trans10

F− mcrA, Δ(mrr-hsdRMS-mcrBC), φ80, lacZΔM15, ΔlacX74, recA1, araΔ139, Δ(ara-leu)7697, galU, galK, rpsL(StrR), endA1, nupG

Transgen Biotech

P. pastoris

 GS115

his4 -

Invitrogen

 JQ01

Derived from GS115, the promoter of IPS gene was replaced with pGAP

This work

 JQ02

Derived from JQ01, with PpITR1 and PpITR2 genes deleted

This work

 JQ03

Derived from JQ02, with pfk2 gene deleted

This work

 JQ04

Derived from JQ03, overexpression of ScIPS gene from S. cerevisiae and EcIMP gene from E. coli

This work

 JQ05

Derived from JQ04, the promoter of zwf gene was replaced with pGUT1

This work

 JQ06

Derived from JQ04, the promoter of pgi gene was replaced with pGUT1

This work

 JQ07

Derived from JQ04, the promoters of pgi and zwf genes were replaced with pGUT1

This work

 JQ08

Derived from JQ07, the promoter of pfk1 gene was replaced with pGUT1

This work

Plasmids

 pGAPZ A

ColE1 ori, ZeoR, pGAP

Invitrogen

 pPIC9K

ColE1 ori, pAOX1 promoter, PpHIS4, AmpR, KanR

Invitrogen

 pEASY-T3

ColE1 ori, AmpR, TA cloning vector

Transgen Biotech

 pJQ

pEASY-T3 derivative; ZeoR, pAOX-mazF

This work

 pJQ01

pJQ derivative for markerless replacing the promoter of IPS gene with pGAP

This work

 pJQ02-T1

pJQ derivative for markerless deletion of possible inositol transporter gene PpITR1

This work

 pJQ02-T2

pJQ derivative for markerless deletion of possible inositol transporter gene PpITR2

This work

 pJQ03

pJQ derivative for markerless deletion of pfk2 gene

This work

 pJQ04

pJQ derivative for markerless knock‑in of ScIPS and EcIMP expression cassettes in the PpITR1 locus

This work

 pJQ05

pJQ derivative for markerless replacing the promoter of zwf gene with pGUT1

This work

 pJQ06

pJQ derivative for markerless replacing the promoter of pgi gene with pGUT1

This work

 pJQ08

pJQ derivative for markerless replacing the promoter of pfk1 gene with pGUT1

This work