Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity

Table 2 Assembly metrics of fosmid DNA sequencing for inserts in clones 88-1G and 102-5A

Fosmid	Sequencing method	De novo assembly program	Total bases	Total reads	Total reads after quality filtering	Total bases in scaffolds	Scaffold N50	Scaffolds No	After filtering & trimming				Number of scaffolds with RAST annotated PEGs
Fosmid	Sequencing method	De novo assembly program	Total bases	Total reads	Total reads after quality filtering	Total bases in scaffolds	Scaffold N50	Scaffolds No	Scaffolds No	Bases No	N50	PEGs No	Number of scaffolds with RAST annotated PEGs
88-1G	Illumina MiSeq: 300 bp paired—end read (Illumina MiSeq V3)	De novo assembly with CLC Genomics Workbench v 8.0	72,271,200	240,904	240,836	506,285	1,284	369	19	16,457	2,564	22	6
102-5A	Illumina MiSeq: 300 bp paired—end read (Illumina MiSeq V3)	De novo assembly with CLC Genomics Workbench v 8.0	73,839,600	246,132	246,080	1,213,995	1,478	800	8	17,793	12,037	21	2

ISSN: 1475-2859