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Table 4 Simulation of metabolic flux of the NADH generation/consumption reaction of C. acetobutylicum ATCC 824 strain under the different conditions

From: Oxidoreduction potential controlling for increasing the fermentability of enzymatically hydrolyzed steam-exploded corn stover for butanol production

Reaction

36 h

60 h

SG

OGG

UCG

SG

OGG

UCG

Flux

Percentage

Flux

Percentage

Flux

Percentage

Flux

Percentage

Flux

Percentage

Flux

Percentage

EMP10

19.01

94.47

12.43

94.81

14.42

78.25

12.35

74.56

9.84

76.88

6.99

75.38

EMP18

0.41

2.03

0.45

3.4

2.48

13.44

3.39

20.48

2.63

20.53

1.94

20.88

BUTAN1

− 0.26

− 1.28

− 1.03

− 8

1.37

7.41

− 0.36

− 2.19

− 0.35

− 2.75

− 0.32

− 3.43

BUTAN2

− 0.22

− 1.09

− 1.01

− 7.81

− 0.88

− 4.76

− 0.35

− 2.10

− 0.84

− 6.53

− 0.41

− 4.38

BUTAN6

− 4.13

− 20.54

− 3.11

− 24.08

− 5.44

− 29.55

− 3.95

− 23.84

− 2.83

− 22.13

− 2.08

− 22.40

BUTAN8

− 8.26

− 41.08

− 6.21

− 48.17

− 10.89

− 59.10

− 6.80

− 41.03

− 4.70

− 36.72

− 3.57

− 38.52

BUTAN11

− 3.52

− 17.49

− 0.7

− 5.44

− 0.56

− 3.01

− 0.55

− 3.32

− 0.48

− 3.78

− 0.29

− 3.14

BUTAN12

− 3.52

− 17.49

− 0.7

− 5.44

− 0.56

− 3.01

− 0.15

− 0.88

− 0.63

− 4.96

− 0.20

− 2.11

TCA2

− 0.07

− 0.33

− 0.04

− 0.33

− 0.03

− 0.17

0.16

0.94

0.00

0.02

0.02

0.25

VLI12

0.07

0.36

0.03

0.36

0.03

0.19

0.04

0.23

0.04

0.27

0.02

0.26

HIS9

0.02

0.12

0.02

0.12

0.01

0.06

0.01

0.08

0.01

0.09

0.01

0.09

HIS10

0.02

0.12

0.02

0.12

0.01

0.06

0.00

0.00

0.00

0.00

0.00

0.00

PTT18

0.13

0.66

0.07

0.68

0.06

0.35

0.00

0.00

0.00

0.00

0.00

0.00

PUR27

0.03

0.17

0.02

0.17

0.02

0.09

0.15

0.91

0.02

0.15

0.05

0.59

PYRM4

0.05

0.26

0.02

0.26

0.03

0.14

0.03

0.17

0.03

0.20

0.02

0.19

FOLATE16

− 0.14

− 0.72

− 0.09

− 0.73

− 0.07

− 0.38

0.06

0.34

0.05

0.40

0.04

0.38

EMP16

0

0

0

0

0

0

− 9.29

− 56.09

− 7.99

− 62.41

− 5.42

− 58.43

GST9

0

0

0

0

0

0

0.38

2.28

0.18

1.41

0.18

1.95

LIMPIN3

0

0

0

0

0

0

0.00

0.03

0.00

0.03

0.00

0.03

PL6

0

0

0

0

0

0

− 0.11

− 0.65

− 0.10

− 0.78

− 0.07

− 0.74

TCA7

0

0

0

0

0

0

− 0.10

− 0.58

− 0.09

− 0.69

− 0.06

− 0.66

  1. The unit of the flux was mmol/g/h
  2. SG the synthetized medium group, OCG detoxicated SECS medium with ORP control at − 350 mV, UCG detoxicated SECS medium without ORP control (UCG)