Fig. 4
Repetitive batches for the production of propanol from propanal with soluble ScADH and BsGDH-CatIBs for NADH recycling. a Measured propanal concentration with GC. b NADH concentration was measured with a photometer. BsGDH-CatIBs, purified from 0.5 mL cells with OD600nm 20, were reused for 11 cycles. 11.5 U ScADH were freshly added with 40 mM TAE (pH 7), 200 mM glucose, 20 mM propanal, 0.4 mM NAD in each cycle. The final reaction volume was 0.5 mL. Each reaction was performed at 37 °C for 30 min. GDH + ADH samples were measured in technical triplicates