Fig. 3

Storage stability testing of BsGDH-PT-CBDCell. The wet pellets (WP) were directly frozen after purification or first lyophilized (LP) before storing them in the freezer at – 20 °C. Technical triplicates were tested. The enzyme assays were performed at 37 °C, 15 min, 1000 rpm, 1 mL in 40 mM TAE buffer (pH 7) with 200 mM glucose and 0.4 mM NAD⁺. A reference assay was performed at day 0, before freezing or freeze-drying. The other assays were performed at day 9, 17, 31, 39, 44 and 56