Fig. 2
From: Hot isopropanol quenching procedure for automated microtiter plate scale 13C-labeling experiments
Combined results of the spiking experiment for validation (bar diagrams) and the isotopically instationary ILE as a proof of concept (line diagrams) accompanied by a simplified portrayal of the metabolic network of C. glutamicum ATCC 13032. The numbers in brackets pertain to each amino acid’s number of carbon atoms. In both experiments, the cultivation began on unlabeled d-glucose but in the former case, U13C d-glucose was added to the quenching reagent, and in the latter case, U13C d-glucose was pulsed before the transient sampling