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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Hot isopropanol quenching procedure for automated microtiter plate scale 13C-labeling experiments

Fig. 1

Complete experimental workflow of the automated cultivation, sacrifice sampling, quenching, and sample processing. 1) A pre-heating step for the hot isopropanol quenching is initiated upon exceeding the cultivation time threshold (tthreshold), whereas the start of the sampling procedure is dependent on crossing a backscatter threshold (BSthreshold). 2) The time for heating the isopropanol solution and the residence time of cellular material inside the heated vials depend on the pipetting scheme of the particular variant of the workflow so the given values are to be understood as lower practical limits. Depending on the subsequent analyses, the extracts may be stored in a sealed deep well plate (a) or Eppendorf tubes (b).

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