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Table 4 The success rate of isolation methods in MBB medium

From: Lichen cell factories: methods for the isolation of photobiont and mycobiont partners for defined pure and co-cultivation

Method

Inoculates

Successful isolations

Success rate [%]

Ascospore

46

30a

65.21%

Thallus Yamamoto

210

0

0.0%

Thallus (this study)

64

12

18.75%

Soredia

48

25

52%

Conidia

26

no conidia discharged

Algae

151

7

4.6%

  1. aAscospores were only discharged in 3 species, which lowered the success rate