From: Active secretion of a thermostable transglutaminase variant in Escherichia coli
Solvent type | Solvent concentration (v/v) (%) | Relative activity (%) | |
---|---|---|---|
FRAP-TGm1 | FRAPD-TGm1 | ||
DMSO | 10 | 87.9 ±  + 2.44 | 84.8 ± 0.71 |
15 | 79.8 ± 1.73 | 79.1 ± 2.54 | |
20 | 70.7 ± 2.00 | 74.8 ± 2.46 | |
25 | 63.8 ± 2.66 | 65.7 ± 2.66 | |
30 | 59.3 ± 2.25 | 47.4 ± 1.44 | |
Ethanol | 10 | 71.2 ± 0.80 | 75.2 ± 1.56 |
15 | 67.9 ± 1.35 | 71.5 ± 2.83 | |
20 | 63.2 ± 1.14 | 67.2 ± 0.60 | |
25 | 59.3 ± 1.38 | 65.2 ± 2.89 | |
30 | 53.0 ± 0.62 | 60.0 ± 1.80 | |
DMF | 10 | 70.1 ± 3.96 | 67.3 ± 1.68 |
15 | 64.0 + 2.22 | 64.2 ± 1.55 | |
20 | 58.3 ± 3.54 | 62.4 ± 2.12 | |
25 | 53.4 ± 2.38 | 53.6 ± 1.08 | |
30 | 50.3 ± 2.71 | 48.6 ± 3.56 | |
Methanol | 10 | 86.8 ± 1.32 | 92.0 ± 3.71 |
15 | 84.0 ± 1.56 | 90.2 ± 2.59 | |
20 | 83.9 ± 2.50 | 87.9 ± 1.58 | |
25 | 81.9 ± 1.71 | 86.1 ± 1.19 | |
30 | 77.4 ± 3.11 | 83.3 ± 1.67 |