Fig. 4
From: Active secretion of a thermostable transglutaminase variant in Escherichia coli
The activation cure of smTG and its variants. Residual activity of smTG variants under 60 ℃ incubation. FRAP-smTG and its variants were expressed in E. coli, which carrying plasmid pET-22b/pro-smTG, pET-22b/pro-TGm1, and pETDuet-1/TrxA-proH-TGm1/TAMEP. The fermentation temperature was under 20 ℃ after induction by adding IPTG at a final concentration of 0.1 mM. Recombinant proteins were purified by affinity purification using the His-Trap column and SEC, which finally eluted in Tris–HCl (50 mM, pH 8.0)