Fig. 2
From: Active secretion of a thermostable transglutaminase variant in Escherichia coli
The effect of co-expressing TAMEP and chaperones on the active expression of TGm1 in E. coli. A The expression cassette for co-expressing TrxA-proH-TGm1 and TAMEP; B The intracellular smTG activity of the E. coli strains with or without co-expressing TAMEP. 1. E. coli expressing pETDuet-1; 2: E. coli carrying pETDuet-1/TrxA-proH-TGm1; 3: E. coli carrying pETDuet-1/TrxA-proH-TGm1/TAMEP. C The intracellular smTG activity and D SDS-PAGE analysis of the E. coli strains with or without co-expressing chaperones. 1: E. coli carrying pETDuet-1/TrxA-proH-TGm1/TAMEP; 2: E. coli carrying pETDuet-1/TrxA-proH-TGm1/TAMEP and pTf16; 3: E. coli carrying pETDuet-1/TrxA-proH-TGm1/TAMEP and pKJE7. Single and double red arrows indicate the positions of mature TGm1 and TrxA-proH-TGm1 bands, respectively. E SDS-PAGE analysis of the TGm1 purified from the intracellular fraction of E. coli carrying pETDuet/TrxA-proH-TGm1/TAMEP and pKJE7 using Nickel affinity chromatography. Each recombinant E. coli was cultivated in TB medium at 20 ℃ for 32 h after 0.1 mM IPTG addition