Table 2 Vectors used in this study
Name | Description | References |
|---|---|---|
Vector | Â | Â |
 pBBR1MCS | A broad-host-range vector, gifted from Prof. Tsuda, Grad. Sch. of Life Science, Tohoku Univ. (GenBank U02374) | Kovach et al. 1994 [42] |
 pHA020_2 | A smaller plasmid in Halomonas sp. A020 (Accession No. AP022852) | Tsuji et al. 2021 [18] |
 pHA020_1 | A larger plasmid in Halomonas sp. A020 (Accession No. AP022851) | Tsuji et al. 2021 [18] |
 pUC19 | A cloning vector (GenBank M77789) | Yanisch-Perron et al. 1985 [43] |
 pG-KJE8 | A chaperone plasmid containing cmr, purchased from Clontech (TaKaRa Bio) | Nishihara et al. 2000 [44] |
 YEp13 | A YE type Saccharomyces cerevisiae—E. coli shuttle vector containing tetr (GenBank U03498) | Broach 1979 [45] |
 pEGFP | A bacterial expression vector containing EGFP tag, purchased from Clontech (TaKaRa Bio) | Inouye et al. 1994 [46] |
 pTrc99a | A bacterial expression vector with lacIq regulated trc promoter (GenBank M22744) | Amann et al. 1988 [47] |
 pwtCas9-bacteria | A Tet-inducible expression vector of Streptococcus pyogenes cas9 gene, purchased from Addgene (Addgene plasmid # 44,250) | Qi et al. 2013 [48] |
 pgRNA-bacteria | An expression vector of Cas9 guide RNA for bacterial gene disruption. purchased from Addgene (Addgene plasmid # 44,251) | Qi et al. 2013 [48] |
KM-1 vector | Â | Â |
 pUCpHAw | pUC19 derivate including whole pHA020_2 and cat/cmr | This work |
 pHA1AT_32 | pUC19 derivate including an origin region of pHA020_1 and tetr under a cat promoter | This work |
Gene expression vector | Â | Â |
 pUCpHAw_EGFP | pUCpHAw derivate including EGFP gene at a PstI site of the pUCpHAw | This work |
 pUCpHAw_Phcp_EGFP | pUCpHAw_EGFP derivate including a promoter region of hcp gene | This work |
 pUCpHAw_Pphasin_EGFP | pUCpHAw_EGFP derivate including a promoter region of phasin gene | This work |
 pUCpHAw_Ptrc_EGFP | pUCpHAw_EGFP derivate including trc promoter with lacIq of pTrc99a | This work |
 pCmHAw_Phcp_zwf | pUCpHAw_Phcp_EGFP derivate substituted EGFP gene to zwf gene | This work |
 pCmHAw_Phcp_phaA | pUCpHAw_Phcp_EGFP derivate substituted EGFP gene to phaA gene | This work |
 pCmHAw_Phcp_tesB | pUCpHAw_Phcp_EGFP derivate substituted EGFP gene to tesB gene | This work |
 pCmHAw_Phcp_pyrE | pUCpHAw_Phcp_EGFP derivate substituted EGFP gene to pyrE gene | This work |
 pCmHAw_Phcp_pyrF | pUCpHAw_Phcp_EGFP derivate substituted EGFP gene to pyrF gene | This work |
Gene disruption vector | Â | Â |
 pTHA(Cas9) | pUCpHAw derivate including a DNA fragment containing lacIq and cas9 under a trc promoter | This work |
 pgRNAHA_pyrF | pHA1AT_31 derivate including a guide RNA for pyrF gene | This work |
 pgRNAHA | pHA1AT_32 derivate including an original guide RNA of pgRNA-bacteria | This work |