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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Establishment of genetic tools for genomic DNA engineering of Halomonas sp. KM-1, a bacterium with potential for biochemical production

Fig. 1

Construction of shuttle vectors. A Two plasmid DNAs were detected in the Halomonas sp. A020. B, C Large and small plasmids in A020 were named pHA020_1 and pHA020_2, respectively. Six and one genes were identified and annotated in pHA020_1 and pHA020_2, respectively. Annotations of the genes are listed in Table 1. Locations of primers to clone the predicted replication origins are indicated with black and white triangles. D Gene map of the first shuttle vector, pUCpHAw, 5239 bp in length. The cmr and Pcat indicate chloramphenicol-resistant gene (cmr/cat) and its promoter from pG-KJE8, respectively. pHA020_2 shows the entire region of pHA020_2. Ec_ori and ampr are E. coli ori and an ampicillin-resistant gene from pUC19, respectively. All vectors are listed in Table 2 and the detailed vector construction is shown in Additional file 1: Fig. S1. Black and white triangles indicate positions of primers corresponding to panel B. E) Gene map of the second shuttle vector, pHA1AT_32, 7061 bp in length. The cmr and pHA020_2 in the pUCpHAw were substituted with a tetracycline-resistant gene (tetr) from YEp13 and the replication origin in the pHA020_1, respectively. The detailed vector construction is shown in Additional file 1: Fig. S2. Black and white triangles indicate positions of primers corresponding to panel C

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