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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Engineering a suite of E. coli strains for enhanced expression of bacterial polysaccharides and glycoconjugate vaccines

Fig. 4

SDS-PAGE of lipid extracts from E. coli strains recombinantly expressing S. pneumoniae capsule. Lipid extracts were separated by SDS–PAGE on a Bolt™ 4–12% bis–tris gel run with MES buffer. A Silver stain; B immunoblot where glycan was detected using anti-serotype 4 primary antibody and anti-rabbit fluorescent secondary antibody. M molecular weight marker PageRuler Plus. Presence or absence of pB4 recombinant S. pneumoniae serotype 4 capsule and waaL are denoted below the lanes as ± respectively, as are the strain names

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Microbial Cell Factories

ISSN: 1475-2859

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