Fig. 1

Engineering a family of glycoengineering strains. The strains are all produced from the progenitor E. coli strain W3110. Subsequent CRISPR mutations are made as designated in the key. Genes in parentheses denote integrations whilst Δ indicates deletions. Strain names are shown above the boxes, which are arranged in a family tree to denote the background strain where successive mutations were introduced, in a series of generations denoted on the left hand side (F1-F5). Strains equivalent to known glycoengineering strains CLM24, CLM37 and SDB1 are also indicated at the side of the boxes in parentheses