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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Efficient D-allulose synthesis under acidic conditions by auto-inducing expression of the tandem D-allulose 3-epimerase genes in Bacillus subtilis

Fig. 1

Enzymatic properties of purified DPEases and non-enzymic browning under alkaline conditions. A SDS-PAGE analysis. Lane 1: Control; lane 2: SDS supernatant; lane 3: SDS precipitation; lane 4: SRC supernatant; lane 5: SRC precipitation; lane 6: SDS-RC supernatant; lane 7: SDS-RC precipitation; lane 8: SRC-DS Supernatant; lane 9: SRC-DS precipitation; lane 10: purified DSDPEase; lane 11 purified RCDPEase. The target proteins were marked by a red box. B Effects of temperature on the activity of DEPases. C The thermostability of DEPases with 0.1 mM Co2+. D Effects of pH on the activity of DEPases. E The pH stability of DPEases with 0.1 mM Co2+. F Effects of metal ions on enzyme activity of DPEases. G The effects of pH on the browning of the reaction solution

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