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Table 2 The specific activities of 7β-hydroxylase, NADK and G6PDH in recombinant Mycolicibacterium neoaurum strains

From: One-pot biosynthesis of 7β-hydroxyandrost-4-ene-3,17-dione from phytosterols by cofactor regeneration system in engineered mycolicibacterium neoaurum

Strains

Enzyme activity

7β-hydroxylase

NADK

G6PDH

(U g− 1)

(U g− 1)

(U g− 1)

M3-261

0

1.24 ± 0.06

1.37 ± 0.07

M3-BM3

0

1.17 ± 0.04

1.31 ± 0.12

M3-mBM3

1.42 ± 0.13

1.12 ± 0.07

1.25 ± 0.04

M3-mBM3-0

2.34 ± 0.11

1.08 ± 0.09

1.18 ± 0.05

M3-mBM3-0-NADK2

2.57 ± 0.08

2.56 ± 0.12

1.21 ± 0.15

M3-mBM3-0-G6PDH2

2.72 ± 0.12

1.24 ± 0.05

3.42 ± 0.13

M3-mBM3-0-NADK2-G6PDH2

3.03 ± 0.06

2.35 ± 0.15

3.34 ± 0.09

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Microbial Cell Factories

ISSN: 1475-2859

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