Fig. 5

The effect of different fermentation conditions on production of 7β-OH-AD. A Effects of different biomass of M3-mBM3-0-NADK2-G6PDH2 (6%, 9%, 12%, 15%) on 7β-OH-AD production. B Effects of different substrate concentrations (1 g L^− 1, 3 g L^− 1, 5 g L^− 1, 7 g L^− 1) on the conversion rate. C Effects of adding time of glucose (3 d, 4 d, 5 d, 6 d) on conversion rate. D Effects of concentrations of glucose supplement (10 g L^− 1, 20 g L^− 1, 30 g L^− 1, 40 g L^− 1) on conversion rate. All assays were performed in triplicate with three independent measurements. Standard deviations of the biological replicates are represented by error bars