Table 1 Strains and plasmids used in this study
From: AdpA, a developmental regulator, promotes ε-poly-l-lysine biosynthesis in Streptomyces albulus
Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
Strains | ||
S. albulus | ||
NK660 | Wild type (WT), ε-poly-l-lysine producer | [34] |
SET | Wild-type strain carrying pSET152 | This work |
NKA | Wild-type strain carrying pSET152-adpASa | This work |
SDA | Wild-type strain carrying pSET152-adpASd | This work |
SHA | Wild-type strain carrying pSET152-adpA-SH | This work |
SNA | Wild-type strain carrying pSET152-adpASn | This work |
SCA | Wild-type strain carrying pSET152-adpA-C | This work |
E. coli | ||
DH5α | General cloning host | TaKaRa |
BL21(DE3) | Host for expression of AdpASn | TransGen Biotech |
WM6026 | Donor strain for conjugation between E. coli and Streptomyces | [57] |
Plasmids | ||
pSET152 | Integrative vector based on ϕC31 integrase | [58] |
pSET152-adpASa | pSET152 derivative for overexpression of adpASa | This work |
pSET152-adpASd | pSET152 derivative for expression of adpASd | This work |
pSET152-adpA-SH | pSET152 derivative for expression of adpA-SH | This work |
pSET152-adpASn | pSET152 derivative for expression of adpASn | This work |
pSET152-adpA-C | pSET152 derivative for expression of adpA-C | This work |
pET-28a ( +) | E. coli expression vector | Novagen |
pET-SNA | adpASn cloned in pET-28a ( +) | This work |