Fig. 1
From: Regulation of heterologous subtilin production in Bacillus subtilis W168
Structure of subtilin and the biosynthesis gene cluster. A Mature subtilin contains 32 amino acids indicated in three-letter code. The lanthionine (Ala-S-Ala) and β-methyl-lanthionine (Abu-S-Ala) bridges are in green. Dehydrated residues are in orange, with Dha represents didehydroalanine and Dhb didehydrobutyrine. B The spa-locus (spaBTCSIFEGRK, ∼12 kb) includes 10 genes that are transcribed in four transcriptional units. The promoters for each transcriptional unit are indicated with grey arrows. PspaS precedes the structural gene spaS; PspaB leads spaBTC operon which encodes proteins involved in the modification and export of subtilin prepeptide; PspaI controls immunity genes spaIFEG for self-protection of subtilin-producing strain; PspaR drives the expression of spaRK for two-component regulatory system SpaRK. The schematics are modified from [45] and not drawn to scale