Fig. 1

Expression and Purification of His-GST-VP1 from E.coli, A His-GST-VP1 purified using nickel chelation chromatography analyzed on 8% SDS-PAGE. Lane 1 represents the protein markers, and lanes 2–4 represent protein fractions eluted with 350 mM imidazole. B Elution profile of His-GST-VP1 from a Superdex 200 (10/300) size exclusion column. C Purified protein from size exclusion chromatography analyzed on 10% SDS-PAGE. Lane 1 represents the protein markers, and lane 2 represents the peak fraction. D Western blots showing the cross reactivity of the purified protein with anti-His (left) and anti-GST (right) antibodies. E and F shows His-GST-VP1 multimers visualized by negative stain and cryo transmission electron microscopy respectively. G shows a 2D class average of His-GST-VP1 multimers from cryo-micrographs