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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Protein purification strategies must consider downstream applications and individual biological characteristics

Fig. 5

Strategies to alleviate protein aggregation. The factors leading to protein aggregation during purification can be mitigated. Operational time should be reduced as much as possible and samples should be kept at low temperatures throughout the entire purification process. Oxidating conditions, high protein concentrations and destabilizing pH values and salt concentrations should be avoided. Mechanical stress needs to be limited and, when necessary, stabilizing molecules should be added. Some proteins may also have specific requirements for reducing agents and detergents

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