Table 1 The soluble level of Notch2NL(1–210) and nClu fused with different tags
From: Retro-protein XXA is a remarkable solubilizing fusion tag for inclusion bodies
Fusion tag (sequence length) | Solubility of fusion protein (%) | |
|---|---|---|
Notch2NL(1–210) | nClu | |
Sumo (98 aa) | 24 | No obvious expression |
Trx (109 aa) | 18 | No obvious expression |
GST (220 aa) | 43 | 26 |
XXA(192 aa) | 94 | 86 |
MBP(357 aa) | 36 | 58 |
NusA(495 aa) | 76 | 18 |