Fig. 9
From: Retro-protein XXA is a remarkable solubilizing fusion tag for inclusion bodies
Conjecture diagram of the principle solubilizing effect of XXA. The aggregation tendency of the target inclusion body protein is prevented by strong Coulomb repulsion of the XXA oligomer. Therefore, the fusion protein cannot aggregate. As a result, amyloid structures cannot form because the aggregation of the target protein is prevented. The target protein can fold correctly in the water phase, significantly improving the soluble expression of the inclusion bodies