Fig. 6
From: Retro-protein XXA is a remarkable solubilizing fusion tag for inclusion bodies
Several inclusion bodies that had enhanced soluble expression by XXA could bind the Ni2+ resin and be purified. a The purified result of fusion protein XXA-bdNEDP1. b The purified result of fusion protein XXA-Notch2NL(1–210). c The purified result of fusion protein XXA-sumo-NbALFA. d The purified result of fusion protein XXA-Chrono(115–306). e The purified result of fusion protein XXA-CUA63106. M marker, U uninduced whole cells, I induced whole cells, S supernatant of the induced cells, P precipitate of the induced cells, Ft flow through, Wash wash the impurity, Elu elution