Fig. 4
From: Retro-protein XXA is a remarkable solubilizing fusion tag for inclusion bodies
Either locate at N-terminal or C-terminal, XXA could promote the soluble expression of heterologous protein. a bdNEDP1 is an inclusion body when expressed alone at 16 °C. b bdNEDP1 was expressed in soluble form at 16 °C and was primarily located in solution after fusion with an N terminal XXA. c bdNEDP1 was expressed as soluble at 16 °C and primarily located in solution after fusion with a C terminal XXA. M marker, U uninduced whole cells, I induced whole cells, S supernatant of the induced cells, P precipitate of the induced cells