Fig. 1
From: Retro-protein XXA is a remarkable solubilizing fusion tag for inclusion bodies
XXA and AXX have a strong solubilizing effect on different inclusion bodies, but XXA is more stable and easier to express. a The fusion proteins were inclusion bodies when Trx-Notch2NL(1–210), GST-Chrono(115–306), and NusA-nClu expression were at 16 °C. b XXA as a fusion tag clearly enhanced the soluble expression of Notch2NL(1–210), Chrono(115–306), and nClu at 16 °C. c AXX could improve the soluble expression of Notch2NL(1–210) and nClu, but AXX-Chrono(115–306) fusion protein could not express the same vector and situation. d The degradation of XXA was slower than that of AXX. M marker, U uninduced whole cells, I induced whole cells, S supernatant of the induced cells, P precipitate of the induced cells