Biosensor-driven, model-based optimization of the orthogonally expressed naringenin biosynthesis pathway

Table 2 Used enzymes for the construction of the naringenin biosynthesis pathway (library)

Abbrev	Organism of origin	Function	EC	Source	References
RgTAL	Rhodotorula glutinis	Tyrosine ammonia-lyase	4.3.1.23	(19)	[19, 22, 63,64,65]
FjTAL	Flavobacterium johnsoniae	Tyrosine ammonia-lyase	4.3.1.23	This study	[66]
Pc4CL	Petroselinum crispum	4-coumaroyl-CoA ligase	6.2.1.12	(19)	[19, 22, 63, 67, 68]
At4CL	Arabidopsis thaliana	4-coumaroyl-CoA ligase	6.2.1.12	iGEM2014:BBa_K1497016	[21]
PhCHS	Petunia hybrida	Chalcone synthase	2.3.1.74	(19)	[19, 22, 63, 67, 68]
GhCHS	Gerbera hybrida	Chalcone synthase	2.3.1.74	iGEM2014:BBa_K1497016	[69]
MsCHI	Medicago sativa	Chalcone isomerase	5.5.1.6	(19)	[19, 22, 63, 68]
PhCHI	Petunia hybrida	Chalcone isomerase	5.5.1.6	iGEM2014:BBa_K1497016	[67, 68]

In addition, their organism origin, function, enzyme classification numbers (EC), source and references are given. The DNA sequence of FjTAL, synthesized in this study, is given in Additional file 1: Table S2.

ISSN: 1475-2859