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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Biosensor-driven, model-based optimization of the orthogonally expressed naringenin biosynthesis pathway

Fig. 3

Overview of the naringenin production screening, strain selection and characterization process. A A strain harboring the heterologous sigma factor B (σB) from Bacillus subtilis in the genome (inserted in the rpoS operon) [8], was cotransformed with the naringenin biosynthetic pathway library and the naringenin-responsive biosensor plasmid (pSynSens1.100) [36]. The strains are screened for naringenin production on microtiter plate-scale through their biosensor-generated fluorescence. B 35 strains were selected that are predicted to cover a wide range of naringenin production titers. These strains were further characterized individually using UPLC analysis to measure the actual naringenin product titers and sequence analysis was used to reveal the incorporated promoters (PB1 to PB10) and coding sequences (CDS) of the isozymes. The characteristics of all 35 strains together with the measured fluorescence/OD600 are given in Additional file 1: Table S1. (Rg: Rhodotorula glutinis; Fj: Flavobacterium johnsoniae; Pc: Petroselinum crispum; At: Arabidopsis thaliana; Ph: Petunia hybrida; Gh: Gerbera hybrida; Ms: Medicago sativa; TAL: Tyrosine ammonia-lyase; 4CL: 4-coumaroyl-CoA ligase; CHS: Chalcone synthase; CHI: Chalcone isomerase)

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