Fig. 2

Construction of the LplttR knockout strain and its effects of on bacterial growth. A Schematic diagram of the LplttR knockout principle. Ha-1 and Ha-2 represent the upstream and downstream homologous arms respectively. The black arrows indicate the location of colony PCR primers used for verification. B Identification of LplttR knockout by colony PCR; C Effect of the LpLttR mutation on bacterial growth. Error bars indicate the standard deviations of three independent experiments