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Table 2 Orthogonal experiment design

From: Enhancing extracellular production of lipoxygenase in Escherichia coli by signal peptides and autolysis system

Experiment number

LOX induction time (h)

(A)

Induction temperature (°C)

(B)

Arabinose concentration (g·L−1)(C)

Blank

(D)

Cell density (OD600)

1

4

25

0.2

0

0.332 ± 0.05

2

4

30

0.6

0

0.214 ± 0.02

3

4

37

1.8

0

0.436 ± 0.04

4

5

25

0.6

0

0.302 ± 0.01

5

5

30

1.8

0

0.413 ± 0.06

6

5

37

0.2

0

1.134 ± 0.08

7

6

25

1.8

0

0.201 ± 0.03

8

6

30

0.2

0

0.869 ± 0.08

9

6

37

0.6

0

1.343 ± 0.09

Km1a

0.327

0.278

0.778

  

Km2a

0.616

0.499

0.620

  

Km3a

0.804

0.971

0.350

  

Smb

0.477

0.693

0.428

  
  1. aKmx is the average targeting value of each factor and can be expressed as Kmx = Gmx/kx, where x (x = 1, 2, 3, 4, 5, 6) and m (m = A, B, C) are the level number and the factor, respectively, Gmx is the sum of the targeting indexes of all levels in each factor m, and kx is the total level of the corresponding factor
  2. bThe range of factor Sm is calculated using Sm = max(Kmx) − min(Kmx)