Skip to main content

Table 2 Orthogonal experiment design

From: Enhancing extracellular production of lipoxygenase in Escherichia coli by signal peptides and autolysis system

Experiment number LOX induction time (h)
(A)
Induction temperature (°C)
(B)
Arabinose concentration (g·L−1)(C) Blank
(D)
Cell density (OD600)
1 4 25 0.2 0 0.332 ± 0.05
2 4 30 0.6 0 0.214 ± 0.02
3 4 37 1.8 0 0.436 ± 0.04
4 5 25 0.6 0 0.302 ± 0.01
5 5 30 1.8 0 0.413 ± 0.06
6 5 37 0.2 0 1.134 ± 0.08
7 6 25 1.8 0 0.201 ± 0.03
8 6 30 0.2 0 0.869 ± 0.08
9 6 37 0.6 0 1.343 ± 0.09
Km1a 0.327 0.278 0.778   
Km2a 0.616 0.499 0.620   
Km3a 0.804 0.971 0.350   
Smb 0.477 0.693 0.428   
  1. aKmx is the average targeting value of each factor and can be expressed as Kmx = Gmx/kx, where x (x = 1, 2, 3, 4, 5, 6) and m (m = A, B, C) are the level number and the factor, respectively, Gmx is the sum of the targeting indexes of all levels in each factor m, and kx is the total level of the corresponding factor
  2. bThe range of factor Sm is calculated using Sm = max(Kmx) − min(Kmx)