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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhancing extracellular production of lipoxygenase in Escherichia coli by signal peptides and autolysis system

Fig. 3

Different extracellular proteins produced by E. coli autolysis. A Time profile of cell growth after inducing cell lysis of autolytic E. coli that expressed different proteins. B Fermentation broth before and after cell lysis. C Extracellular enzyme activity after cell lysis. The red bar represents the intracellular enzyme activity after sonication. The green bar represents the extracellular enzyme activity by cell autolysis. D The expression level of extracellular protein after cell lysis. Lane 1 represents whole cells fraction; lanes 4 and 7 represent intracellular fraction; lanes 2, 5, and 8 represent fermentation broth supernatant; and lanes 3 and 6 represent cell debris after lysis. The arrow indicates the target protein

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Microbial Cell Factories

ISSN: 1475-2859

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