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Table 5 Vectors used in this study

From: Production of indole by Corynebacterium glutamicum microbial cell factories for flavor and fragrance applications

Strain Description Source
pGold KmR, PtrclacIq, pGA1, oriVEc; C. glutamicum/E. coli expression shuttle vector with BsaI recognition site for Golden Gate assembly [30]
pK19mobsacB KmR, E. coli/C. glutamicum shuttle vector for construction of insertion and deletion mutants in C. glutamicum (pK18 oriVEc sacB lacZα) [59]
pACYCDuet-1 CmR, P15A (pACYC184 replicon), encodes two multiple cloning sites, each of which is preceded by a T7 promoter, a lac operator, and a ribosome binding site Novagen
pGold-EctnaA pGold expressing tnaA from E. coli MG1655 This work
pGold-EctnaA-EctnaB pGold expressing tnaA and tnaB from E. coli MG1655 This work
pGold-EctnaA-CgaroP pGold expressing tnaA from E. coli MG1655 and aroP from C. glutamicum ATCC13032 This work
pGold-HstnaA pGold expressing tnaA from Histophilus somni This work
pGold-MitnaA pGold expressing tnaA from Mageeibacillus indolicus This work
pGold-SstnaA pGold expressing tnaA from Synechocystis sp. This work
pGold-PrtnaA pGold expressing tnaA from Proteiniclasticum ruminis This work
pGold-CxtnaA pGold expressing tnaA from Corynebacterium xerosis This work
pGold-EhtnaA pGold expressing tnaA from Escherichia hermannii This work
pGold-CbtnaA pGold expressing tnaA from Clostridiales bacterium This work
pGold-PretnaA pGold expressing tnaA from Providencia rettgeri This work
pGold-PptnaA pGold expressing tnaA from Prevotella pallens This work
pGold-SmtnaA pGold expressing tnaA from Sciscionella marina This work
pGold-NstnaA pGold expressing tnaA from Nocardioides sp. This work
pGold-RstnaA pGold expressing tnaA from Rhodobacter sphaeroides This work
pGold-PstnaA pGold expressing tnaA from Photobacterium sp. This work
pGold-PvtnaA pGold expressing tnaA from Proteus vulgaris This work
pGold-HstnaA-CgaroP pGold expressing tnaA from Histophilus somni and aroP from C. glutamicum ATCC13032 This work
pGold-MitnaA-CgaroP pGold expressing tnaA from Mageeibacillus indolicus and aroP from C. glutamicum ATCC13032 This work
pGold-PrtnaA-CgaroP pGold expressing tnaA from Proteiniclasticum ruminis and aroP from C. glutamicum ATCC13032 This work
pGold-PretnaA-CgaroP pGold expressing tnaA from Providencia rettgeri and aroP from C. glutamicum ATCC13032 This work
pGold-PptnaA-CgaroP pGold expressing tnaA from Prevotella pallens and aroP from C. glutamicum ATCC13032 This work
pGold-PvtnaA-CgaroP pGold expressing tnaA from Proteus vulgaris and aroP from C. glutamicum ATCC13032 This work
pGold-PretnaA_artRBS-CgaroP pGold expressing tnaA from Providencia rettgeri with artificial RBS and aroP from C. glutamicum ATCC13032 This work
pGold-PretnaA-EcridA-CgaroP pGold expressing tnaA from Providencia rettgeri with artificial RBS, ridA from E. coli MG1655 and aroP from C. glutamicum ATCC13032 This work
pGold-PretnaA-EcridA-CgaroP pGold expressing tnaA from Providencia rettgeri with artificial RBS, yjgH from E. coli MG1655 and aroP from C. glutamicum ATCC13032 This work
pK19- Δpyk pK19mobsacB derivative with the flanking region of pyk [44]
pACYCDuet-EctnaA pACYCDuet-1 expressing tnaA from E. coli MG1655 This work
pACYCDuet-MitnaA pACYCDuet-1 expressing tnaA from Mageeibacillus indolicus This work
pACYCDuet-PretnaA pACYCDuet-1 expressing tnaA from Providencia rettgeri This work
pACYCDuet-PvtnaA pACYCDuet-1 expressing tnaA from Proteus vulgaris This work