Table 5 Vectors used in this study
Strain | Description | Source |
|---|---|---|
pGold | KmR, PtrclacIq, pGA1, oriVEc; C. glutamicum/E. coli expression shuttle vector with BsaI recognition site for Golden Gate assembly | [30] |
pK19mobsacB | KmR, E. coli/C. glutamicum shuttle vector for construction of insertion and deletion mutants in C. glutamicum (pK18 oriVEc sacB lacZα) | [59] |
pACYCDuet-1 | CmR, P15A (pACYC184 replicon), encodes two multiple cloning sites, each of which is preceded by a T7 promoter, a lac operator, and a ribosome binding site | Novagen |
pGold-EctnaA | pGold expressing tnaA from E. coli MG1655 | This work |
pGold-EctnaA-EctnaB | pGold expressing tnaA and tnaB from E. coli MG1655 | This work |
pGold-EctnaA-CgaroP | pGold expressing tnaA from E. coli MG1655 and aroP from C. glutamicum ATCC13032 | This work |
pGold-HstnaA | pGold expressing tnaA from Histophilus somni | This work |
pGold-MitnaA | pGold expressing tnaA from Mageeibacillus indolicus | This work |
pGold-SstnaA | pGold expressing tnaA from Synechocystis sp. | This work |
pGold-PrtnaA | pGold expressing tnaA from Proteiniclasticum ruminis | This work |
pGold-CxtnaA | pGold expressing tnaA from Corynebacterium xerosis | This work |
pGold-EhtnaA | pGold expressing tnaA from Escherichia hermannii | This work |
pGold-CbtnaA | pGold expressing tnaA from Clostridiales bacterium | This work |
pGold-PretnaA | pGold expressing tnaA from Providencia rettgeri | This work |
pGold-PptnaA | pGold expressing tnaA from Prevotella pallens | This work |
pGold-SmtnaA | pGold expressing tnaA from Sciscionella marina | This work |
pGold-NstnaA | pGold expressing tnaA from Nocardioides sp. | This work |
pGold-RstnaA | pGold expressing tnaA from Rhodobacter sphaeroides | This work |
pGold-PstnaA | pGold expressing tnaA from Photobacterium sp. | This work |
pGold-PvtnaA | pGold expressing tnaA from Proteus vulgaris | This work |
pGold-HstnaA-CgaroP | pGold expressing tnaA from Histophilus somni and aroP from C. glutamicum ATCC13032 | This work |
pGold-MitnaA-CgaroP | pGold expressing tnaA from Mageeibacillus indolicus and aroP from C. glutamicum ATCC13032 | This work |
pGold-PrtnaA-CgaroP | pGold expressing tnaA from Proteiniclasticum ruminis and aroP from C. glutamicum ATCC13032 | This work |
pGold-PretnaA-CgaroP | pGold expressing tnaA from Providencia rettgeri and aroP from C. glutamicum ATCC13032 | This work |
pGold-PptnaA-CgaroP | pGold expressing tnaA from Prevotella pallens and aroP from C. glutamicum ATCC13032 | This work |
pGold-PvtnaA-CgaroP | pGold expressing tnaA from Proteus vulgaris and aroP from C. glutamicum ATCC13032 | This work |
pGold-PretnaA_artRBS-CgaroP | pGold expressing tnaA from Providencia rettgeri with artificial RBS and aroP from C. glutamicum ATCC13032 | This work |
pGold-PretnaA-EcridA-CgaroP | pGold expressing tnaA from Providencia rettgeri with artificial RBS, ridA from E. coli MG1655 and aroP from C. glutamicum ATCC13032 | This work |
pGold-PretnaA-EcridA-CgaroP | pGold expressing tnaA from Providencia rettgeri with artificial RBS, yjgH from E. coli MG1655 and aroP from C. glutamicum ATCC13032 | This work |
pK19- Δpyk | pK19mobsacB derivative with the flanking region of pyk | [44] |
pACYCDuet-EctnaA | pACYCDuet-1 expressing tnaA from E. coli MG1655 | This work |
pACYCDuet-MitnaA | pACYCDuet-1 expressing tnaA from Mageeibacillus indolicus | This work |
pACYCDuet-PretnaA | pACYCDuet-1 expressing tnaA from Providencia rettgeri | This work |
pACYCDuet-PvtnaA | pACYCDuet-1 expressing tnaA from Proteus vulgaris | This work |