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Table 2 Overview of indole production upon several optimization strategies

From: Production of indole by Corynebacterium glutamicum microbial cell factories for flavor and fragrance applications

Strain

Media components

Indole determination (sampling time)

Trp conversion

Carbon source

Nitrogen source

Trp

Indole

C1*(pGold-PretnaA-CgaroP)

40 g L−1 glucose

20 g L−1 (NH4)2SO4

5 g L−1 urea

1 g L−1

–

0.35 ± 0.01 g L−1 (24 h)

0.25 ± 0.01 g L−1 (48 h)

61 mol-% (24 h)

44 mol-% (48 h)

C1*(pGold-PretnaA-CgaroP)

40 g L−1 gluconate

20 g L−1 (NH4)2SO4

5 g L−1 urea

1 g L−1

–

0.21 ± 0.01 g L−1 (24 h)

37 mol-%

C1*(pGold-PretnaA-CgaroP)

10 g L−1 glucose

20 g L−1 (NH4)2SO4

5 g L−1 urea

1 g L−1

–

0.45 ± 0.01 g L−1 (24 h)

0.44 ± 0.01 g L−1 (48 h)

78 mol-% (24 h)

77 mol-% (48 h)

C1*(pGold-PretnaA-CgaroP)

10 g L−1 glucose

5 g L−1 (NH4)2SO4

1.25 g L−1 urea

1 g L−1

–

0.51 ± 0.01 g L−1

89 mol-%

C1*(pGold-PretnaA-CgaroP)

10 g L−1 glucose

5 g L−1 (NH4)2SO4

1.25 g L−1 urea

2 g L−1

–

0.77 ± 0.01 g L−1 (24 h)

0.91 ± 0.01 g L−1 (48 h)

67 mol-% (24 h)

79 mol-% (48 h)

C1*(pGold-PretnaA-CgaroP)

10 g L−1 glucose

5 g L−1 (NH4)2SO4

1.25 g L−1 urea

2 g L−1 (0 h)

2 g L−1 (24 h)

–

0.94 ± 0.01 g L−1 (48 h)

41 mol-%

C1* Δpyk (pGold-PretnaA-CgaroP)

40 g L−1 glucose

20 g L−1 (NH4)2SO4

5 g L−1 urea

1 g L−1

–

0.28 ± 0.01 g L−1

49 mol-%

C1* Δpyk (pGold-PretnaA-CgaroP)

40 g L−1 gluconate

1 g L−1 glucose

20 g L−1 (NH4)2SO4

1.25 g L−1 urea

1 g L−1

–

0.18 ± 0.01 g L−1

31 mol-%

C1*(pGold-PretnaA_artRBS-CgaroP)

10 g L−1 glucose

5 g L−1 (NH4)2SO4

1.25 g L−1 urea

1 g L−1

–

0.38 ± 0.01 g L−1

66 mol-%

C1*(pGold-PretnaA-EcridA-CgaroP)

10 g L−1 glucose

5 g L−1 (NH4)2SO4

1.25 g L−1 urea

1 g L−1

–

0.37 ± 0.01 g L−1

66 mol-%

C1*(pGold-PretnaA-EcyjgH-CgaroP)

10 g L−1 glucose

5 g L−1 (NH4)2SO4

1.25 g L−1 urea

1 g L−1

–

0.37 ± 0.01 g L−1

66 mol-%

IVO20 (pGold-PretnaA-CgaroP)

10 g L−1 glucose

5 g L−1 (NH4)2SO4

1.25 g L−1 urea

2 g L−1 (0 h)

2 g L−1 (24 h)

–

0.68 ± 0.01 g L−1 (48 h)

30 mol-% (48 h)

IVO38 (pGold-PretnaA-CgaroP)

10 g L−1 glucose

5 g L−1 (NH4)2SO4

1.25 g L−1 urea

2 g L−1 (0 h)

2 g L−1 (24 h)

–

0.63 ± 0.01 g L−1 (48 h)

27 mol-% (48 h)

  1. When not specified otherwise, media components were added with inoculation and indole was determined in culture supernatants 24 h after inoculation