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Table 2 Overview of indole production upon several optimization strategies

From: Production of indole by Corynebacterium glutamicum microbial cell factories for flavor and fragrance applications

Strain Media components Indole determination (sampling time) Trp conversion
Carbon source Nitrogen source Trp Indole
C1*(pGold-PretnaA-CgaroP) 40 g L−1 glucose 20 g L−1 (NH4)2SO4
5 g L−1 urea
1 g L−1 0.35 ± 0.01 g L−1 (24 h)
0.25 ± 0.01 g L−1 (48 h)
61 mol-% (24 h)
44 mol-% (48 h)
C1*(pGold-PretnaA-CgaroP) 40 g L−1 gluconate 20 g L−1 (NH4)2SO4
5 g L−1 urea
1 g L−1 0.21 ± 0.01 g L−1 (24 h) 37 mol-%
C1*(pGold-PretnaA-CgaroP) 10 g L−1 glucose 20 g L−1 (NH4)2SO4
5 g L−1 urea
1 g L−1 0.45 ± 0.01 g L−1 (24 h)
0.44 ± 0.01 g L−1 (48 h)
78 mol-% (24 h)
77 mol-% (48 h)
C1*(pGold-PretnaA-CgaroP) 10 g L−1 glucose 5 g L−1 (NH4)2SO4
1.25 g L−1 urea
1 g L−1 0.51 ± 0.01 g L−1 89 mol-%
C1*(pGold-PretnaA-CgaroP) 10 g L−1 glucose 5 g L−1 (NH4)2SO4
1.25 g L−1 urea
2 g L−1 0.77 ± 0.01 g L−1 (24 h)
0.91 ± 0.01 g L−1 (48 h)
67 mol-% (24 h)
79 mol-% (48 h)
C1*(pGold-PretnaA-CgaroP) 10 g L−1 glucose 5 g L−1 (NH4)2SO4
1.25 g L−1 urea
2 g L−1 (0 h)
2 g L−1 (24 h)
0.94 ± 0.01 g L−1 (48 h) 41 mol-%
C1* Δpyk (pGold-PretnaA-CgaroP) 40 g L−1 glucose 20 g L−1 (NH4)2SO4
5 g L−1 urea
1 g L−1 0.28 ± 0.01 g L−1 49 mol-%
C1* Δpyk (pGold-PretnaA-CgaroP) 40 g L−1 gluconate
1 g L−1 glucose
20 g L−1 (NH4)2SO4
1.25 g L−1 urea
1 g L−1 0.18 ± 0.01 g L−1 31 mol-%
C1*(pGold-PretnaA_artRBS-CgaroP) 10 g L−1 glucose 5 g L−1 (NH4)2SO4
1.25 g L−1 urea
1 g L−1 0.38 ± 0.01 g L−1 66 mol-%
C1*(pGold-PretnaA-EcridA-CgaroP) 10 g L−1 glucose 5 g L−1 (NH4)2SO4
1.25 g L−1 urea
1 g L−1 0.37 ± 0.01 g L−1 66 mol-%
C1*(pGold-PretnaA-EcyjgH-CgaroP) 10 g L−1 glucose 5 g L−1 (NH4)2SO4
1.25 g L−1 urea
1 g L−1 0.37 ± 0.01 g L−1 66 mol-%
IVO20 (pGold-PretnaA-CgaroP) 10 g L−1 glucose 5 g L−1 (NH4)2SO4
1.25 g L−1 urea
2 g L−1 (0 h)
2 g L−1 (24 h)
0.68 ± 0.01 g L−1 (48 h) 30 mol-% (48 h)
IVO38 (pGold-PretnaA-CgaroP) 10 g L−1 glucose 5 g L−1 (NH4)2SO4
1.25 g L−1 urea
2 g L−1 (0 h)
2 g L−1 (24 h)
0.63 ± 0.01 g L−1 (48 h) 27 mol-% (48 h)
  1. When not specified otherwise, media components were added with inoculation and indole was determined in culture supernatants 24 h after inoculation