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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Production of indole by Corynebacterium glutamicum microbial cell factories for flavor and fragrance applications

Fig. 5

In situ product recovery enabled production of indole in g per liter scale. C1*(pGold-PretnaA-CgaroP) was cultivated in CGXII supplemented with 10 g L−1 glucose, 2 g L−1 Trp and 20% (v/v) dibutyl sebacate. Production titer at 3 h, 6 h and 9 h is shown. 24 h+: indole accumulation in dibutyl sebacate, when additional 2 g L−1 Trp were supplemented 9 h after inoculation. 24 h ++: indole accumulation in dibutyl sebacate, when additional 8 g L−1 Trp were supplemented 9 h after inoculation. Indole concentrations were determined in solvent layer by HPLC exclusively, since aqueous phase was not enriched by the same. Indole concentrations were normalized to the volume of the medium phase, as they were 5-times concentrated in the overlay phase. Means and arithmetic errors of duplicate cultures are shown. A HPLC chromatograms of the overlay of the cultivation 24 h ++ recorded at 270 nm and of an indole standard (Ind) are shown (B)

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