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Table 1 Primer sequences used in qPCR amplification

From: Study on the mechanism of efficient extracellular expression of toxic streptomyces phospholipase D in Brevibacillus choshinensis under Mg2+ stress

Gene

Primer

Sequence (5′–3′)

phospholipase D

phospholipase D-F

GGCGTGGGCATCAAGGAGT

 

phospholipase D-R

CGGCGTTGGTGTTGTCGTG

HWP

HWP-F

CTCGCTTTGACTATGTACTGG

 

HWP-R

TTTGGTGCCGTGACTACTTC

16S rRNA

16S rRNA-F

TCGTGTCGTGAGATGTTG

 

16S rRNA-R

CCTTCCTCCGTCTTGTC