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Table 1 Strains and plasmids that were used in this study

From: Engineering microbial consortia of Elizabethkingia meningoseptica and Escherichia coli strains for the biosynthesis of vitamin K2

Strains/plasmids

Relevant characteristic(s)

Source

Strains

 E. coli DH5α Competent Cells

F-φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(r k−,m k+) supE44λ- thi-1 gyrA96 relA1 phoA

Sangon Biotech

 E. coli BL21(DE3) Competent Cells

F- ompT hsdSB(rB-mB-) gal dcm(DE3)

Sangon Biotech

 Elizabethkingia meningoseptica sp. F2

The original strain was obtained from China Center for Type Culture Collection (CCTCC) AB2010137. Mutagenic strains

Our lab

 J01

E. coli BL21 carrying PETA-1

This study

 J02

E. coli BL21 carrying PETD-2

This study

 H01

E. coli BL21 carrying PETA-2, PACD-2, PETD-2

This study

 H02

E. coli BL21 carrying PETA-3, PACD-2, PETD-2

This study

 H03

E. coli BL21 carrying pET28a-GFP, PETD-2

This study

Co-cultivation system

 CO1

Co-culture of E. meningoseptica sp. F2 and E. coli J02

This study

 CO2

Co-culture of E. meningoseptica sp. F2 and E. coli H01

This study

 CO3

Co-culture of E. meningoseptica sp. F2 and E. coli H02

This study

 CO4

Co-culture of E. meningoseptica sp. F2 and E. coli H03

This study

Plasmids

 pET28a

T7 promoter, KanR

Novagen

 pET28a-GFP

pET28a carrying GFP, KanR

Novagen

 pACYCDuet-1

double T7 promoters, CmR

Novagen

 pETDuet™-1

double T7 promoters, Amp

Novagen

 PETD-1

pETDuetâ„¢-1 carrying MBP-EmGGPPS, Amp

This study

 PETD-2

pETDuetâ„¢-1 carrying MBP-EmGGPPS-GB1EmOPPS, Amp

This study

 PETA-1

pET28a carrying GB1-EmOPPS-His, Kan

This study

 PETA-2

pET28a carrying ScePMK-EmPVD, Kan

This study

 PETA-3

pET28a carrying ScePMK-EmPVD-EcMenA-EmMenE, Kan

This study

 PACD-1

pACYCDuet-1 carrying EmHMGS-EmHMGR, CmR

This study

 PACD-2

pACYCDuet-1 carrying EmHMGS-EmHMGR-SceMK-EmIDI, CmR

This study

  1. Elizabethkingia meningoseptica sp. F2 was obtained from the China Center for Type Culture Collection. The ID should be CCTCC AB2010137 instead of CCTCC AB2011070 [30]