Fig. 6

Expression and identification of AA9 LPMOs of thermophilic fungi in the horse gut. A Expression of AA9 LPMOs in the horse stomach using RT-PCR: Selected AA9 LPMOs are CtPMO1, TaAA9A, and HiPMO1. Amplified PCR products were sequenced and confirmed to be CtPMO1 and TaAA9A but HiPMO1 was not observed. B Identification of AA9 LPMOs of thermophilic fungi using SDS-PAGE: Proteins from the horse gut were isolated by ion-exchange chromatography on a DEAE-sepharose column. Isolated proteins were visualized by staining with Coomassie Brilliant Blue. M, protein marker (170, 130, 95, 72, 55, 43, 34, 26 kDa); Fractions of proteins from DEAE-sepharose column were shown in 10, 11, 12, 13, 14, 15, and 16, respectively. C Identification of AA9 LPMOs of thermophilic fungi using LC-MS/MS: Isolated proteins were digested with trypsin. The resulting peptides matched to AA9 LPMOs from thermophilic fungi were shown in red. The singal peptide sequences were shown in blue