Fig. 5

The growth and AA9 LPMO expression of the three thermophilic fungi on cellulose-containing medium at 37 °C. A The three isolated thermophilic fungi were cultured on cellulose-containing agar medium at 37 °C for 7 days. B Expression of AA9 LPMOs from the three isolated thermophilic fungi at 37 °C on cellulose-containing medium for 7 days using RT-PCR. Selected AA9 LPMOs are HiPMO1 from S. thermophilum, CtPMO1 from C. thermophilum, and TaAA9A from T. aurantiacus. The amplified PCR products were sequenced and confirmed to be HiPMO1, CtPMO1, and TaAA9A