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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Oxidative cleavage of cellulose in the horse gut

Fig. 3

Identification of C1- and C4-oxidized products in the soluble oligosaccharides extracted from the horse stomach using MALDI-TOF-MS and HPLC-RID. A MALDI-TOF-MS: The extracted soluble oligosaccharides were permethylated with methyl iodide. C1-oxidized oligosaccharides (m/z + 30), C4-oxidized oligosaccharides (m/z − 16), non-oxidized oligosaccharides (m/z + 0). B HPLC-RID of C1-oxidized products: C1-oxidized products are hydrolyzed by TFA to glucose and gluconic acid. S: the soluble oligosaccharides; CK, control sample analyzed as above except without the soluble oligosaccharides; Standard, a mixture of glucose and gluconic acid. C HPLC-RID of C4-oxidized products: C4-oxidized products soluble cello-oligosaccharides are reduced by NaBH4 followed by hydrolysis with TFA to yield glucose, galactose, and sorbitol. Standard, a mixture of glucose, galactose, and sorbitol; S: the soluble oligosaccharides; CK, the control sample analyzed as above except without the soluble oligosaccharides

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