Fig. 2
Identification of C1-oxidized products in the digested cellulose isolated from horse stomach using MALDI-TOF-MS and HPLC-RID. A MALDI-TOF-MS: The digested cellulose was hydrolyzed by endocellulase followed by permethylation with methyl iodide. C1-oxidized cello-oligosaccharides (m/z + 30), non-oxidized cello-oligosaccharides (m/z + 0). Some molecular ion peaks with CH3OH occur in MALDI-TOF-MS spectra. B HPLC-RID: The digested cellulose was hydrolyzed by endocellulase followed by hydrolysis with TFA. C1-oxidized products are hydrolyzed by TFA to glucose and gluconic acid. Standard, a mixture of glucose and gluconic acid; S: The digested cellulose; CK, the control sample analyzed as above except without the digested celllulose