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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Oxidative cleavage of cellulose in the horse gut

Fig. 1

Identification of C1-oxidized products of the digested cellulose isolated from fresh horse feces. A LC-MS: The digested cellulose hydrolyzed by endocellulase followed by hydrolysis with TFA. LC-MS showing extraction chromatograms and corresponding mass spectra of gluconic acid. In positive mode: gluconic acid (m/z 196 + H+). B HPAEC-PAD: The digested cellulose hydrolyzed by endocellulase followed by hydrolysis with TFA. S, the digested cellulose; CK, the control sample analyzed as above except without the digested celllulose; Standards, glucose, and gluconic acid. C HPLC-RID: The digested cellulose was hydrolyzed by endocellulase followed by hydrolysis with TFA. C1-oxidized products are hydrolyzed by TFA to glucose and gluconic acid. Standard, a mixture of glucose and gluconic acid; S: the digested cellulose; CK, the control sample analyzed as above except without the digested cellulose. D MALDI-TOF-MS: The digested cellulose hydrolyzed by endocellulase followed by permethylation with methyl iodide. C1-oxidized cello-oligosaccharides (m/z + 30), non-oxidized cello-oligosaccharides (m/z + 0). Endocellulase reaction products permethylated by methyl iodide were dissolved in methanol, and molecular ion peaks of CH3OH occur in MALDI-TOF-MS spectra

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