Table 2 primers used for endolysin cloning
Endolysin gene | Cloning method | PCR primers |
|---|---|---|
MatN | RFC | Fw 5′-GGATCGGAAAACCTGTATTTTCAGGGATCCATGGAGGTGGCGACAATG-3′ |
Rv 5′-GGTGGCTCCAGCTGCCGGATCCCTAACTGAT TTCTCCCCATAAGTCA-3′ | ||
Cg | RFC | Fw 5′-GGATCGGAAAACCTGTATTTTCAGGGATCCGTAATGGCTAAGACTCAAGCAG-3′ |
Rv 5′-GTGGCTCCAGCTGCCGGATCCCTATTTGAATACTCCCCAGGCA-3′ | ||
MatN | RD + L | Fw 5′-GCGCATATGCAAGCAAAACTAACTA-3′ |
Rv 5′-GGATCCGAGATTTCTCCCCATAAGTC-3′ |