Endolysin | Description | Expression vector/E. coli strain | Induction conditions | Lysis buffer | Purification yields (mg/L of bacterial culture) |
---|---|---|---|---|---|
LysH5 [18] | Endolysin from phage ΦH5: N-terminal CHAP, a central amidase domain (N-acetylmuramyl-l-alanine amidase), and a C-terminal SH3b CBD | pRSETB (N-ter His-tag)/BL21(DE3)/pLys | 1 mM IPTG/18 h—19 °C | 20 mM NaH2PO4,500 mM NaCl, 20 mM Imidazole, pH 7.4 | 2 mg/L |
Lys-phiSA012 [27] | endolysin from phage phiSA012: N-terminal CHAP, a central amidase domain (N-acetylmuramyl-l-alanine amidase), and a C-terminal SH3b CBD | pGEX-6P-2 (C-ter GST-tag)/BL21(DE3) | 0.1 mM IPTG/O.N.—25 °C | 50 mM Tris–HCl, 1 M MgCl2 and 10% NP-40 | Not stated |
plyGRCS [28] | endolysin from phage GRCS: N-terminal both CHAP and endopeptidase activity and a C-terminal SH3b CBD | pBAD24 (C-His-tag)/BL21 (DE3) | 0,25% ara/O.N.—18 °C | Not stated | Not stated |
LysGH15 [17] | Endolysin from phage GH15: N-terminal CHAP, a central amidase domain (N-acetylmuramyl-l-alanine amidase), and a C-terminal SH3b CBD | pET15b (N-ter His-tag)/BL21 (DE3) (Codon Plus) | 1 mM IPTG/6 h—25 °C | 20 mM PBS | Not stated |
2638A [29] | Endolysin from phage 2638A: N-terminal M23 peptidase domain, a mid-protein amidase 2 domain, and a C-terminal SH3b_5 (SH3b) CBD | pET21a (C-ter His-tag)/BL21(DE3) | 1 mM IPTG/18 h—10 °C | Not stated | Not stated |
ClyS [15] | Quimeric endolysin: N-terminal catalytic domain of Twort phage endolysin and C-terminal CBD from phi13 phage endolysin | pJML6/DH5α | 2% lactose/O.N.—30 °C | 20 mM NaH2PO4, 1 mM DTT | Not stated |
CHAP-AMIDASE [30] | Quimeric endolysin: N-terminal CHAP domain and C-terminal amidase-2 domain from Phage K endolysin (LysK). GS-His tag linker | pET-22b (pelB leader)/BL21 (DE3) | 1 mM IPTG/4 h—37 °C | Not stated | 8–12 mg/L |
LysP108 [31] | Endolysin from phage P108: N-terminal amidase and C-terminal SH3b CBD | pET21a (C-ter His-tag)/BL21 (DE3) | 0,1 mM IPTG/10 h—23 °C | Not stated | Not stated |
LysDW2 [32] | Endolysin from phage DW2: N-terminal CHAP, a central amidase domain (N-acetylmuramyl-l-alanine amidase), and a C-terminal SH3b CBD | pQE-60 (C-ter His-tag)/XL1Blue | 1 mM IPTG/5 h—37 °C | BugBuster® protein extraction reagent, Millipore, Merk | Not stated (only CHAP truncated domain was soluble) |
SAL-1 [33] | endolysin from phage SAP-1: SAL-1 differs from LysK at three residues (ILE- > VAL 26, GLN- > GLU 114, and HIS- > GLN 486) | pBAD-TOPO (no His-tag)/BL21 (DE3) | 0.2 mM ara/10 h—19 °C | 50 mM Na2HPO4 (pH 7.5), 10 mM EDTA, 1 mM DTT | 80 mg/L |
LysCSA13 [26] | endolysin from phage CSA13: N-terminal CHAP and C-terminal SH3-5 CBD | pET28a (N-ter His-tag)/BL21 (DE3) | 0,5 mM IPTG/20 h–18 °C | 50 mM NaH2PO4, 300 mM NaCl, pH 8.0 | 10 mg/L |