Fig. 1

MatN and Cg domain architecture and alignment with reference endolysins. Prediction of the domain architecture was analyzed using InterPro. All endolysins are composed of a CHAP (cysteine, histidine-dependent amidohydrolase/peptidase; green), Ami (Amidase; red) and SH3b (blue) domain. Multiple alignment analysis of our endolysins MatN and Cg with endolysins previously reported in the bibliography (LysK, LysGH15 and LysH5) was done using Clustal Omega. Identical residues are shaded in gray and increasing degree of residue conservation correlates with darkening of the gray scale of the bar above the sequences